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Double-stranded DNA can be denatured by heating and renatured by cooling Renaturation under conditions of high stringency has which of the following effects?
o A) Considerable base mismatching is permitted
o B) GC base pairing is inhibited
o C) GT base pairing is promoted
o D) Mismatched DNA hybridization is prevented
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can u expalin this please
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well, dna renaturation is an imp step in southern blot or pcr. here a well characterized probe dna is added to a target molecule. the mixed samle is denatured and then renatured. when probe dna bind to the target dna sequence of sufficient complimentarity, its hybridization. i studied today, its in kaplan xenetics.
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Answer is D
Since regeneration occurs under high stringency conditions ssDNA will only pair with exact complementary sequence hence mismatching is prevented.
