09-08-2018, 06:28 AM
PCR amplification is carried out on the region containing the CAG repeat, which of the following sets of primers would be used to amplify the CAG repeat in the brackets (CAG)
GAG TCC CTC AAG TCC TTC (CAG) CAA CAG CCG CCA CCG CCG
A. CTC AGG GAG TTC AGG AAG & CGG CGG TGG CGG CTG TTG
B. CTT CCT GAA CTC CCT GAG & CAA CAG CCG CCA CCG CCG
C. CTT CCT GAA CTC CCT GAG & CGG CGG TGG CCG CTG TTG
D. GAG TCC CTC AAG TCC TTC & CGG CGG TGG CGG CTG TTG
E. GAG TCC CTC AAG TCC TTC & GTT GTC GGC GGT GGC GGC
with Explanation plz how to answer such questions as i feel really confused .. thanks
GAG TCC CTC AAG TCC TTC (CAG) CAA CAG CCG CCA CCG CCG
A. CTC AGG GAG TTC AGG AAG & CGG CGG TGG CGG CTG TTG
B. CTT CCT GAA CTC CCT GAG & CAA CAG CCG CCA CCG CCG
C. CTT CCT GAA CTC CCT GAG & CGG CGG TGG CCG CTG TTG
D. GAG TCC CTC AAG TCC TTC & CGG CGG TGG CGG CTG TTG
E. GAG TCC CTC AAG TCC TTC & GTT GTC GGC GGT GGC GGC
with Explanation plz how to answer such questions as i feel really confused .. thanks