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A pharmaceutical firm is interested in the bacterial production of thymidylate synthase in large quantities for drug-targeting studies .An important step in the overall cloning strategy involves the ligation of synthase cDNA into a plasmid vector containing a replication origin ,an antibiotic resistance gene,and a promoter sequence.Which additional nucleotide sequence should be included in this vector to ensure optimal production of the thymidylate synthase ?
A.Operator sequence
B.PolyA sequence
C.Shine-Dalgarno sequence
D.Attenuator sequence
E.3'-splice acceptor sequence
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Could you please post correct answer?
Thank you so much
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Yes C is the correct answer.Here is the explanation as given in the book:
........>incorporation of a Shine-Dalgarno sequence into the expression vector will promote ribosome binding to the translation start site on the mRNA produced by transcription of the cDNA insert
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Thank you so much for your prompt response and the great questions!!!! Also, I would like to say that I have read your story and you should be extremely proud of yourself for all your effort. You are extremely courageous to be back here and fight again and I am 100% sure that you will excel!!!
Of course, your health is most important!!! Good luck!!!!
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C..
RNA...binds to Shine-Dalgarno sequence to initiate translation
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Yes,thank you very much Marymam.
Thank you so much Elpida.
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Sorry for the typing error.Thank you very much Maryam2009 for the input.
