@redceo123 - cardio69

[ArchivalUser]

Let see if you are clear http://i.imgur.com/NK9LGyz.png?1 72sec like always.
Show me yr work.

[ArchivalUser]

DDD

5' TCCTGAGC------------AAATGTGT 3' is given

so the complementary sequence would be 3' AGGACTCG---------TTTACACA 5'

the primer must be complementary to 3' AGGACTCG so it would be 5' TCCTGAGC 3'

AND

the second part is put in 5' to 3' direction.

Hence, answer is 5' TCCTGAGC; 5' ACACATTT 3'


Please be right, please be right. i spent way more than 72 secs but hopefully i got the concept.

[ArchivalUser]

hey sorry if im intruding in but 1 question

i remember sth either from kaplan or DIT if the question doesmt mention 5' end or 3' we should be considering something ;'l*()

but i forgot how to figure it out !!!!!

So please can you tell me with the question cardio has posted :0

[ArchivalUser]

D

should work

[ArchivalUser]

if they dont mention in the question, you assume it is in 5' to 3' direction.

[ArchivalUser]

@red thanks

[ArchivalUser]

*D* RT
WD @red.

[ArchivalUser]

D is the answer

[ArchivalUser]

Can someone please explain the last part of the answer???? please? thats the only thing tripping me up. the last part is running 5' to 3' already so why did we take the complement of it? the first part is running from 5' to 3' and the complement of that was not taken???

[ArchivalUser]

@jaz, Do you understand POLYMERASE CHAIN REX/PCR? and how it works? For region of DNA to be amplified?
I suggest go and read. Come back here and tell me what part you don't understand. It seem you having hard time to understand that.